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Biochemistry

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JoVE Journal Biochemistry
Studie van de functies en activiteiten van Neuronale K-Cl Co-Transporter KCC2 met behulp van Western Blotting
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Studie van de functies en activiteiten van Neuronale K-Cl Co-Transporter KCC2 met behulp van Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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Het huidige protocol benadrukt de toepassing van western blotting-techniek om de functies en activiteiten van neuronale K-Cl co-transporter KCC2 te bestuderen. Het protocol beschrijft het onderzoek naar KCC2-fosforylering op kinaseregelplaatsen Thr906/1007 via western blotting. Ook aanvullende methoden om KCC2-activiteit te bevestigen worden kort gemarkeerd in deze tekst.

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Biochemie Nummer 190
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