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Biochemistry

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JoVE Journal Biochemistry
Zuivering van endogene Drosophila Transient Receptor Potential Channels
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Zuivering van endogene Drosophila Transient Receptor Potential Channels

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Op basis van het assemblagemechanisme van het INAD-eiwitcomplex werd in dit protocol een aangepaste affiniteitszuivering plus concurrentiestrategie ontwikkeld om het endogene Drosophila TRP-kanaal te zuiveren.

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Biochemie eiwitzuivering Drosophila TRP-kanaal INAD
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