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Een Western Blotting Protocol voor kleine aantallen van hematopoietische stamcellen
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Een Western Blotting Protocol voor kleine aantallen van hematopoietische stamcellen

Article DOI: 10.3791/56855-v • 08:45 min • August 22nd, 2018
August 22nd, 2018 •
1,2,3, 1, 2
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University

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Een standaard Western blotting protocol is geoptimaliseerd voor het analyseren van slechts 500 hematopoietische stamcellen of voorlopercellen. Optimalisatie houdt zorgvuldige behandeling van het monster van de cel, beperking van de overdracht tussen buizen en direct het lysing van cellen in Laemmli monster buffer.

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Biochemie kwestie 138 westelijke bevlekken hematopoietische stamcellen kleincellige cijfers SDS-pagina de beperking van dierproeven beenmerg
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