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Verrijking van volwassen muis dorsale wortel ganglia neuronculturen door immunopanning
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Verrijking van volwassen muis dorsale wortel ganglia neuronculturen door immunopanning

Article DOI: 10.3791/64603-v • 08:57 min • February 24th, 2023
February 24th, 2023 •
1, 1,2,3, 1,4,5
1Neuroscience and Mental Health Institute, University of Alberta, 2Department of Medicine, Division of Neurology, University of Alberta, 3Department of Medical Microbiology and Immunology, University of Alberta, 4Department of Pharmacology, University of Alberta, 5Department of Anesthesiology and Pain Medicine, University of Alberta

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Dit artikel beschrijft een immunopanningprotocol voor ganglia van de dorsale wortel van volwassen muizen. Door antilichamen aan kweekplaten te hechten, kunnen we niet-neuronale cellen negatief selecteren en verwijderen. We laten zien dat de culturen zijn verrijkt voor neuronen met behulp van dit protocol, waardoor een diepgaande studie van neuronale reacties op manipulatie mogelijk is.

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