This protocol describes the use of an inertial microfluidics-based buffer exchange strategy to purify micro/nanoparticle engineered cells with efficient depletion of unbound particles.
Tay, H. M., Yeo, D. C., Wiraja, C., Xu, C., Hou, H. W. Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering. J. Vis. Exp. (113), e54327, doi:10.3791/54327 (2016).