Cellular senescence is the key factor in the development of chronic age-related pathologies. Identification of therapeutics that target senescent cells show promise for extending healthy aging. Here, we present a novel assay to screen for the identification of senotherapeutics based on measurement of senescence associated β-Galactosidase activity in single cells.
Fuhrmann-Stroissnigg, H., Santiago, F. E., Grassi, D., Ling, Y., Niedernhofer, L. J., Robbins, P. D. SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs. J. Vis. Exp. (148), e58133, doi:10.3791/58133 (2019).