Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Tyler Mrozowich; Steffane McLennan; Michael Overduin; Trushar R. Patel

doi:10.3791/58538

Dziennik

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Biochemia

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Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

JoVE Journal
Biochemia

This content is Free Access.

JoVE Journal Biochemia

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

12,522 Views

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07:19 min

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November 05, 2018

DOI:

10.3791/58538-v

DOI:

10.3791/58538-v

•

07:19 min

November 05, 2018

•

18 Views

Tyler Mrozowich¹, Steffane McLennan², Michael Overduin^2,4, Trushar R. Patel^1,3,4

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry,University of Lethbridge, ²Department of Biochemistry,University of Alberta, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry,University of Alberta

Transkrypcja

Podsumowanie

Here, we present how Small Angle X-Ray Scattering (SAXS) can be utilized to obtain information on low-resolution envelopes representing the macromolecular structures. When used in conjunction with high-resolution structural techniques such as X-Ray Crystallography and Nuclear Magnetic Resonance, SAXS can provide detailed insights into multidomain proteins and macromolecular complexes in-solution.