Pasteur Institute of Tunis 1 article published in JoVE Biology Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae Elhem Yacoub1, Imen Chniba1, Nadine Khadraoui1, Béhija Mlik1, Boutheina Ben Abdelmoumen Mardassi1 1Group of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Pasteur Institute of Tunis In Mycoplasma pneumoniae infection, serology tests can generate good results, yet with low specificity because of immunological cross-reaction. The in-house antigen-capture ELISA, described in this paper, guarantees high species specificity and has been shown to be a reliable screening test for accurate diagnosis of M. pneumoniae.