Zhejiang Academy of Traditional Chinese Medicine 1 article published in JoVE Medicine Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection Yang Zhang*1, Lifeng Shen*2, Ping'er Wang3, Weixing Xi4, Zhongming Yu1, Xiaowen Huang1, Xuping Wang1, Dan Shou1 1Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, 2Department of Orthopaedic Surgery, Tongde Hospital of Zhejiang Province, 3Institute of Orthopaedics and Traumatology, First Affiliated Hospital of Zhejiang Chinese Medical University, 4Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province This study presents an improved rabbit model infected with Staphylococcus aureus by blocking the same amount of bacteria in bone marrow. Vancomycin loaded calcium sulphate and autogenous bone are used for antibiotic and bone repair treatment. The protocol could be helpful for studying bone infection and regeneration.