The Second Affiliated Hospital of Hainan Medical University 1 article published in JoVE Biology Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice Peng Yu*1, Bo Lin*2, Jingyang Li1, Yanqing Luo1, Di Zhang1, Jiayi Sun1, Xianli Meng1, Yingfan Hu3, Li Xiang1 1State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, 3The School of Preclinical Medicine, Chengdu University Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs.
