Bar-Ilan Institute for Nanotechnology and Advanced Materials (BINA) 1 article published in JoVE Bioengineering Designing Porous Silicon Films as Carriers of Nerve Growth Factor Michal Rosenberg1, Neta Zilony2,3, Orit Shefi2,3, Ester Segal1,4 1Department of Biotechnology and Food Engineering, Technion - Israel Institute of Technology, 2Faculty of Engineering, Bar-Ilan University, 3Bar-Ilan Institute of Nanotechnologies and Advanced Materials, 4Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.