Singapore University of Technology and Design 1 article published in JoVE Bioengineering Polymeric Microneedle Array Fabrication by Photolithography Himanshu Kathuria1, Jaspreet Singh Kochhar1, Michelle Hui Min Fong1, Michinao Hashimoto2, Ciprian Iliescu3, Hanry Yu3,4,5, Lifeng Kang1 1Department of Pharmacy, National University of Singapore, 2Singapore University of Technology and Design, 3Institute of Bioengineering and Nanotechnology, Agency for Science, Technology and Research (A*STAR), 4Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 5Mechanobiology Institute, National University of Singapore Here, we present a protocol describing a mold-free fabrication process of the polymeric microneedles by photolithography.