University of Aveiro 3 articles published in JoVE Neuroscience Optimized Automated Analysis of Live Neuronal Mitochondria Homeostasis Modulation by Isoform-Specific Retinoic Acid Receptors José J. M. Vitória1, Vinícius de Paula1, Odete A. B. da Cruz e Silva1, Diogo Trigo1 1Neuroscience and Signalling Laboratory, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro The mitochondrial network is extremely complex, making it very challenging to analyze. A novel MATLAB tool analyzes live confocal imaged mitochondria in timelapse images but results in a large output volume requiring individual manual attention. To address this issue, a routine optimization was developed, allowing for speedy file analysis. Engineering Development of Efficient OLEDs from Solution Deposition Manish Kumar1,2, Luiz Pereira1 1Department of Physics and i3N, Institute for Nanostructures, Nanomodulation and Nanofabrication, University of Aveiro, 2CeNTI, Centre for Nanotechnologies and Smart Materials Presented here is a protocol for the fabrication of efficient, simple, solution-deposited organic light-emitting diodes with low roll-off. Neuroscience Isolation and Culture of Chick Ciliary Ganglion Neurons Filipa J. Costa*1, Marta S. Dias*1, Rui O. Costa2, Joana R. Pedro2, Ramiro D. Almeida1,2 1Institute of Biomedicine, Department of Medical Sciences - iBiMED, University of Aveiro, 2CNC - Center for Neuroscience and Cell Biology, University of Coimbra Chick ciliary ganglia (CG) are part of the parasympathetic nervous system. Neuronal cultures of chick CG neurons were shown to be effective cell models in the study of nerve muscle interactions. We describe a detailed protocol for the dissection, dissociation and in vitro culture of CG neurons from chick embryos.
