Merck and Co.Inc. 1 article published in JoVE Neuroscience Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications Chloe Ann Whitehouse1, Yufang He2, Janet Brownlees1, Nicola Corbett1 1MSD Research Laboratories, London, UK, 2Merck & Co., Inc., Rahway, NJ, USA Here, we present a protocol to produce 3D-bioprinted cocultures of iPSC-derived neurons and astrocytes. This coculture model, generated within a hydrogel scaffold in 96- or 384-well formats, demonstrates high post-print viability and neurite outgrowth within 7 days and shows the expression of maturity markers for both cell types.