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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Article DOI: 10.3791/2220-v • 10:37 min • December 21st, 2010
December 21st, 2010 •
*1, *1, 1
1Department of Biology, Portland State University
* These authors contributed equally

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We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.

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UV-induced Replication Intermediates E. Coli Two-dimensional Agarose-gel Analysis Inaccurate Replication DNA Damage Cellular Rearrangements Mutagenesis Cancer Development Gene Products Replication Encounters DNA Lesions Replicating Plasmids UV-induced DNA Damage Replication Forks RecA RecF Pathway Nucleotide Excision Repair
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