Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Priyanka Dutta; Christina Lehmann; Devang Odedra; Deepika Singh; Christian Pohl

doi:10.3791/53469

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Rastreamento e quantificação processos de desenvolvimento em C. elegans Como usar as ferramentas de código aberto

Tracking and Quantifying Developmental Processes in <em>C. elegans</em> Using Open-source Tools

JoVE Journal
Biologia

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JoVE Journal Biologia

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Rastreamento e quantificação processos de desenvolvimento em C. elegans Como usar as ferramentas de código aberto

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8,821 Views

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10:41 min

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December 16, 2015

DOI:

10.3791/53469-v

DOI:

10.3791/53469-v

•

10:41 min

December 16, 2015

•

9 Views

Priyanka Dutta*¹, Christina Lehmann*¹, Devang Odedra*¹, Deepika Singh*¹, Christian Pohl¹

¹Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine,Goethe University

Summary

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.