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An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Article DOI: 10.3791/56470-v • 09:32 min • November 8th, 2017
November 8th, 2017 •
1, 2, 3, 2, 2, 1
1Laboratory of Plant Genetics and Development, Noble Research Institute, 2Genetics Laboratory, University of Oklahoma Health Science Center, 3Medicine and Health School, Li Shui University

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This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.

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Array-based Comparative Genomic Hybridization Copy Number Variations Fast Neutron-induced Mutants Medicago Truncatula Legume Biology Deletion Mutations Neutron Bombardment Mutants DNA Isolation Kit Wild Type Genomic DNA Mutant Genomic DNA Random Primer Thermocycler
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