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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Article DOI: 10.3791/57442-v • 11:13 min • April 10th, 2018
April 10th, 2018 •
*1,2, *3, 1,2, 3
1IVI-RMA New Jersey, 2Jefferson College of Biomedical Sciences, Thomas Jefferson University, 3Department of Genetics, Rutgers, The State University of New Jersey
* These authors contributed equally

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As the genetic variants associated with human disease begin to become uncovered, it is becoming increasingly important to develop systems with which to rapidly evaluate the biological significance of those identified variants. This protocol describes methods for evaluating human gene function during female meiosis I using mouse oocytes.

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Mouse Oocytes Human Gene Function Meiosis I Genetic Predisposition Maternal Aneuploidy Mammalian Meiosis Gene Function Studies In Vitro Fertilization Treatments Embryonic Aneuploidy Rates Visual Demonstration Image Analysis Analysis Parameters Cloning Strategy Coding Sequence In Vitro Transcription Vector Mutagenize DNA Single Nucleotide Polymorphisms Insertions And Deletions Bacterial Host Plasmid Purification Restriction Enzyme Digestion
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