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Murine Fecal Isolation and Microbiota Transplantation

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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The goal here is to outline a protocol to investigate the mechanisms of dysbiosis in cardiovascular disease. This paper discusses how to aseptically collect and transplant murine fecal samples, isolate intestines, and use the "Swiss-roll" method, followed by immunostaining techniques to interrogate changes in the gastrointestinal tract.

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Murine Fecal Isolation Microbiota Transplantation Swiss Roll Method Gastrointestinal Tract Cecum Surgical Scissors Sterile Petri Dish Sterile Tubes Aliquots 80 Degrees Celsius Freezer Fecal Pellets Sterile Saline Vortex Homogenized Nylon Filter Centrifuge Supernatant Oral Gavage Germ-free Recipient Mouse Restraints Mouse Holder
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