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Immunology and Infection

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Murine fekal isolasjon og mikrobiotatransplantasjon

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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Målet her er å skissere en protokoll for å undersøke mekanismene for dysbiose ved kardiovaskulær sykdom. Denne artikkelen diskuterer hvordan man aseptisk samler og transplanterer murine fekale prøver, isolerer tarmene og bruker "Swiss-roll" -metoden, etterfulgt av immunfargingsteknikker for å forhøre endringer i mage-tarmkanalen.

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Immunologi og infeksjon utgave 195 fekal mikrobiotatransplantasjon sveitserrulle kardiovaskulær
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