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JoVE Journal Cancer Research
Generering og avbildning av mus og humane epitelorganoider fra normalt og tumorbrystvev uten å passere
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Generering og avbildning av mus og humane epitelorganoider fra normalt og tumorbrystvev uten å passere

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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Denne protokollen diskuterer en tilnærming for å generere epitelorganoider fra primær normal og tumor brystvev gjennom differensial sentrifugering. Videre er instruksjoner inkludert for tredimensjonal dyrking samt immunfluorescerende avbildning av innebygde organoider.

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