Name: Microdissecção Multinível e Perfil Funcional-Estrutural de Ramos Arteriais Renais Humanos (Vídeo) | JoVE Journal
Uploaded: 2025-09-05T13:00:00.000+00:00
Duration: 6 min 51 s
Description: Aprenda a isolar e avaliar funcionalmente ramos arteriais renais humanos usando microdisseção e miografia de arame. Este método permite análise vascular precisa em modelos humanos específicos, superando limitações de espécies animais e melhorando o desenvolvimento translacional de fármacos.

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<ol><li>Molema, G., Aird, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+heterogeneity+in+the+kidney%5BTitle%5D)+AND+%22Semin+Nephrol%22%5BJournal%5D)">Vascular heterogeneity in the kidney.</a> Semin Nephrol. 32 (2), 145-155 (2012).</li>
<li>Jentzer, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Contemporary+management+of+severe+acute+kidney+injury+and+refractory+cardiorenal+syndrome%3A+Jacc+council+perspectives%5BTitle%5D)+AND+%22J+Am+Coll+Cardiol%22%5BJournal%5D)">Contemporary management of severe acute kidney injury and refractory cardiorenal syndrome: Jacc council perspectives.</a> J Am Coll Cardiol. 76 (9), 1084-1101 (2020).</li>
<li>Kanji, H. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Difference+between+preoperative+and+cardiopulmonary+bypass+mean+arterial+pressure+is+independently+associated+with+early+cardiac+surgeryassociated+acute+kidney+injury%5BTitle%5D)+AND+%22J+Cardiothorac+Surg%22%5BJournal%5D)">Difference between preoperative and cardiopulmonary bypass mean arterial pressure is independently associated with early cardiac surgeryassociated acute kidney injury.</a> J Cardiothorac Surg. 5 (1), 71(2010).</li>
<li>Kwiatkowska, E., Kwiatkowski, S., Dziedziejko, V., Tomasiewicz, I., Domanski, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+microcirculation+injury+as+the+main+cause+of+ischemic+acute+kidney+injury+development%5BTitle%5D)+AND+%22Biology+%28Basel%29%22%5BJournal%5D)">Renal microcirculation injury as the main cause of ischemic acute kidney injury development.</a> Biology (Basel). 12 (2), 327(2023).</li>
<li>Ruilope, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hypertension+in+2010%3ABlood+pressure+and+the+kidney%5BTitle%5D)+AND+%22Nat+Rev+Nephrol%22%5BJournal%5D)">Hypertension in 2010:Blood pressure and the kidney.</a> Nat Rev Nephrol. 7 (2), 73-74 (2011).</li>
<li>Barger, A. C., Herd, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+renal+circulation%5BTitle%5D)+AND+%22N+Engl+J+Med%22%5BJournal%5D)">The renal circulation.</a> N Engl J Med. 284 (9), 482-490 (1971).</li>
<li>Beloncle, F., Piquilloud, L., Asfar, P. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aBeloncle%2C+F+%22Critical+care+nephrology%22">Critical care nephrology</a>. , third edition, Elsevier. Philadelphia. (2019).</li>
<li>MompeóCorredera, B., HernándezMorera, P., CastañoGonzález, I., QuintanaMontesdeoca, M. D. P., MederosReal, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Regions+of+the+human+renal+artery%3AHistomorphometric+analysis%5BTitle%5D)+AND+%22Anat+Cell+Biol%22%5BJournal%5D)">Regions of the human renal artery:Histomorphometric analysis.</a> Anat Cell Biol. 55 (3), 330-340 (2022).</li>
<li>Evans, R. G., Smith, D. W., Lee, C. J., Ngo, J. P., Gardiner, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((What+makes+the+kidney+susceptible+to+hypoxia%5BTitle%5D)+AND+%22Anat+Rec+%28Hoboken%29%22%5BJournal%5D)">What makes the kidney susceptible to hypoxia.</a> Anat Rec (Hoboken). 303 (10), 2544-2552 (2020).</li>
<li>Hwa, J. J., Ghibaudi, L., Williams, P., Chatterjee, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+acetylcholinedependent+relaxation+in+large+and+small+arteries+of+rat+mesenteric+vascular+bed%5BTitle%5D)+AND+%22Am+J+Physiol%22%5BJournal%5D)">Comparison of acetylcholinedependent relaxation in large and small arteries of rat mesenteric vascular bed.</a> Am J Physiol. 266 (3 Pt 2), H952-H958 (1994).</li>
<li>Buus, N. H., Van Bavel, E., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differences+in+sensitivity+of+rat+mesenteric+small+arteries+to+agonists+when+studied+as+ring+preparations+or+as+cannulated+preparations%5BTitle%5D)+AND+%22Br+J+Pharmacol%22%5BJournal%5D)">Differences in sensitivity of rat mesenteric small arteries to agonists when studied as ring preparations or as cannulated preparations.</a> Br J Pharmacol. 112 (2), 579-587 (1994).</li>
<li>Barone, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+arterial+and+venous+doppler+in+cardiorenal+syndrome%3A+Pathophysiological+and+clinical+insights%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">Renal arterial and venous doppler in cardiorenal syndrome: Pathophysiological and clinical insights.</a> Biomedicines. 12 (6), 1166(2024).</li>
<li>Cuttone, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exploring+the+utility+of+renal+resistive+index+in+critical+care%3A+Insights+into+ARDS+and+cardiac+failure%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">Exploring the utility of renal resistive index in critical care: Insights into ARDS and cardiac failure.</a> Biomedicines. 13 (2), 519(2025).</li>
<li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Clinical+practice+report+of+contrastenhanced+ultrasound+in+renal+artery+disease%5BTitle%5D)+AND+%22J+Ultrasound+Med%22%5BJournal%5D)">Clinical practice report of contrastenhanced ultrasound in renal artery disease.</a> J Ultrasound Med. 43 (1), 117-125 (2023).</li>
<li>Andersen, F. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Continuous+assessment+of+tissue+perfusion+using+quantitative+indocyanine+green+fluorescence+imaging+during+controlled+hypo+and+reperfusion%5BTitle%5D)+AND+%22Surg+Endosc%22%5BJournal%5D)">Continuous assessment of tissue perfusion using quantitative indocyanine green fluorescence imaging during controlled hypo and reperfusion.</a> Surg Endosc. 39 (10), 6512-6521 (2025).</li>
<li>Schubert, R., Gaynullina, D., Shvetsova, A., Tarasova, O. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myography+of+isolated+blood+vessels%3AConsiderations+for+experimental+design+and+combination+with+supplementary+techniques%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Myography of isolated blood vessels:Considerations for experimental design and combination with supplementary techniques.</a> Front Physiol. 14 (8), 1176748(2023).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mir1865p+carried+by+M2+macrophagederived+exosomes+downregulates+TRPP2+expression+in+airway+smooth+muscle+to+alleviate+asthma+progression%5BTitle%5D)+AND+%22Int+Immunopharmacol%22%5BJournal%5D)">Mir1865p carried by M2 macrophagederived exosomes downregulates TRPP2 expression in airway smooth muscle to alleviate asthma progression.</a> Int Immunopharmacol. 148 (1), 114107(2025).</li>
<li>Ikeda, M., Nakada, A., Abukawa, H., Yamazaki, T., Maruyama, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vibegron+inhibits+enhanced+spontaneous+contractions+induced+by+anoxia%2Freoxygenation+in+isolated+whole+bladder+from+rats%5BTitle%5D)+AND+%22Eur+J+Pharmacol%22%5BJournal%5D)">Vibegron inhibits enhanced spontaneous contractions induced by anoxia/reoxygenation in isolated whole bladder from rats.</a> Eur J Pharmacol. 926 (1), 175017(2022).</li>
<li>Wenceslau, C. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Guidelines+for+the+measurement+of+vascular+function+and+structure+in+isolated+arteries+and+veins%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Guidelines for the measurement of vascular function and structure in isolated arteries and veins.</a> Am J Physiol Heart Circ Physiol. 321 (1), H77-H111 (2021).</li>
<li>Bayliss, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((On+the+local+reactions+of+the+arterial+wall+to+changes+of+internal+pressure%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">On the local reactions of the arterial wall to changes of internal pressure.</a> J Physiol. 28 (3), 220-231 (1902).</li>
<li>Darmon, M., Schnell, D., Schneider, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Monitoring+of+renal+perfusion%5BTitle%5D)+AND+%22Intensive+Care+Med%22%5BJournal%5D)">Monitoring of renal perfusion.</a> Intensive Care Med. 48 (10), 1505-1507 (2022).</li>
<li>Offenhäuser, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((EphA2+regulates+vascular+permeability+and+prostate+cancer+metastasis+via+modulation+of+cell+junction+protein+phosphorylation%5BTitle%5D)+AND+%22Oncogene%22%5BJournal%5D)">EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylation.</a> Oncogene. 44 (4), 208-227 (2025).</li>
<li>Vitverova, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Soluble+endoglin+and+hypercholesterolemia+aggravate+endothelial+and+vessel+wall+dysfunction+in+mouse+aorta%5BTitle%5D)+AND+%22Atherosclerosis%22%5BJournal%5D)">Soluble endoglin and hypercholesterolemia aggravate endothelial and vessel wall dysfunction in mouse aorta.</a> Atherosclerosis. 271, 15-25 (2018).</li>
<li>Maathuis, M. H. J., De Groot, M., Ploeg, R. J., Leuvenink, H. G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deterioration+of+endothelial+and+smooth+muscle+cell+function+in+DCD+kidneys+after+static+cold+storage+in+IGL1+or+UW%5BTitle%5D)+AND+%22J+Surg+Res%22%5BJournal%5D)">Deterioration of endothelial and smooth muscle cell function in DCD kidneys after static cold storage in IGL1 or UW.</a> J Surg Res. 152 (2), 231-237 (2009).</li>
<li>Ingemansson, R., Budrikis, A., Bolys, R., Sjoberg, T., Steen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+temperature+in+longterm+preservation+of+vascular+endothelial+and+smooth+muscle+function%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Effect of temperature in longterm preservation of vascular endothelial and smooth muscle function.</a> Ann Thorac Surg. 61 (5), 1413-1417 (1996).</li>
<li>McIntyre, C. A., Williams, B. C., Lindsay, R. M., McKnight, J. A., Hadoke, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preservation+of+vascular+function+in+rat+mesenteric+resistance+arteries+following+cold+storage%2C+studied+by+small+vessel+myography%5BTitle%5D)+AND+%22Br+J+Pharmacol%22%5BJournal%5D)">Preservation of vascular function in rat mesenteric resistance arteries following cold storage, studied by small vessel myography.</a> Br J Pharmacol. 123 (8), 1555-1560 (1998).</li>
<li>Ingemansson, R., Sjoberg, T., Massa, G., Steen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Longterm+preservation+of+vascular+endothelium+and+smooth+muscle%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Longterm preservation of vascular endothelium and smooth muscle.</a> Ann Thorac Surg. 59 (5), 1177-1181 (1995).</li>
<li>Zheng, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HMGB2+promotes+smooth+muscle+cell+proliferation+through+PPAR%CE%B3%2FPGC1%CE%B1+pathwaymediated+glucose+changes+in+aortic+dissection%5BTitle%5D)+AND+%22Atherosclerosis%22%5BJournal%5D)">HMGB2 promotes smooth muscle cell proliferation through PPARγ/PGC1α pathwaymediated glucose changes in aortic dissection.</a> Atherosclerosis. 399, 119044(2024).</li>
<li>Zhang, B., Zhang, Y., Deng, F., Fang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ligustrazine+prevents+basilar+artery+remodeling+in+twokidneytwoclip+renovascular+hypertension+rats+via+suppressing+PI3K%2FAKT+signaling%5BTitle%5D)+AND+%22Microvasc+Res%22%5BJournal%5D)">Ligustrazine prevents basilar artery remodeling in twokidneytwoclip renovascular hypertension rats via suppressing PI3K/AKT signaling.</a> Microvasc Res. 128, 103938(2020).</li>
<li>Somiari, S. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+the+quality+of+RNA+isolated+from+human+breast+tissue+after+ambient+room+temperature+exposure%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure.</a> PLoS One. 17 (1), e0262654(2022).</li>
<li>Srinivasan, M., Sedmak, D., Jewell, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+fixatives+and+tissue+processing+on+the+content+and+integrity+of+nucleic+acids%5BTitle%5D)+AND+%22Am+J+Pathol%22%5BJournal%5D)">Effect of fixatives and tissue processing on the content and integrity of nucleic acids.</a> Am J Pathol. 161 (6), 1961-1971 (2002).</li>
<li>Potente, M., Makinen, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+heterogeneity+and+specialization+in+development+and+disease%5BTitle%5D)+AND+%22Nat+Rev+Mol+Cell+Biol%22%5BJournal%5D)">Vascular heterogeneity and specialization in development and disease.</a> Nat Rev Mol Cell Biol. 18 (8), 477-494 (2017).</li>
<li>Lake, B. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+atlas+of+healthy+and+injured+cell+states+and+niches+in+the+human+kidney%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">An atlas of healthy and injured cell states and niches in the human kidney.</a> Nature. 619 (7970), 585-594 (2023).</li>
<li>Greenbaum, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+spatially+resolved+timeline+of+the+human+maternal-fetal+interface%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">A spatially resolved timeline of the human maternal-fetal interface.</a> Nature. 619 (7970), 595-605 (2023).</li>
<li>The Tabula Sapiens Consortium. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Tabula+Sapiens%3A+A+multipleorgan%2C+singlecell+transcriptomic+atlas+of+humans%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">The Tabula Sapiens: A multipleorgan, singlecell transcriptomic atlas of humans.</a> Science. 376 (6594), eabl4896(2022).</li>
<li>Barnett, S. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+organotypic+atlas+of+human+vascular+cells%5BTitle%5D)+AND+%22Nat+Med%22%5BJournal%5D)">An organotypic atlas of human vascular cells.</a> Nat Med. 30 (12), 3468-3481 (2024).</li>
<li>Shen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeting+cytokinelike+protein+FAM3D+lowers+blood+pressure+in+hypertension%5BTitle%5D)+AND+%22Cell+Rep+Med%22%5BJournal%5D)">Targeting cytokinelike protein FAM3D lowers blood pressure in hypertension.</a> Cell Rep Med. 4 (6), 101072(2023).</li>
<li>Zhao, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Restoration+of+autophagic+flux+improves+endothelial+function+in+diabetes+through+lowering+mitochondrial+ROSmediated+eNOS+monomerization%5BTitle%5D)+AND+%22Diabetes%22%5BJournal%5D)">Restoration of autophagic flux improves endothelial function in diabetes through lowering mitochondrial ROSmediated eNOS monomerization.</a> Diabetes. 71 (5), 1099-1114 (2022).</li>
<li>Ergin, B., Akin, S., Ince, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Kidney+microcirculation+as+a+target+for+innovative+therapies+in+AKI%5BTitle%5D)+AND+%22J+Clin+Med%22%5BJournal%5D)">Kidney microcirculation as a target for innovative therapies in AKI.</a> J Clin Med. 10 (18), (2021).</li>
<li>Deferrari, G., Cipriani, A., La Porta, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+dysfunction+in+cardiovascular+diseases+and+its+consequences%5BTitle%5D)+AND+%22J+Nephrol%22%5BJournal%5D)">Renal dysfunction in cardiovascular diseases and its consequences.</a> J Nephrol. 34 (1), 137-153 (2021).</li>
<li>Owen, M. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue+potentiates+contraction+of+coronary+vascular+smooth+muscle%3A+Influence+of+obesity%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Perivascular adipose tissue potentiates contraction of coronary vascular smooth muscle: Influence of obesity.</a> Circulation. 128 (1), 9-18 (2013).</li>
<li>AyalaLopez, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue+contains+functional+catecholamines%5BTitle%5D)+AND+%22Pharmacol+Res+Perspect%22%5BJournal%5D)">Perivascular adipose tissue contains functional catecholamines.</a> Pharmacol Res Perspect. 2 (3), e00041(2014).</li>
<li>AyalaLopez, N., Thompson, J. M., Watts, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue%27s+impact+on+norepinephrineinduced+contraction+of+mesenteric+resistance+arteries%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Perivascular adipose tissue's impact on norepinephrineinduced contraction of mesenteric resistance arteries.</a> Front Physiol. 8, 37(2017).</li>
<li>Ahmad, M. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipocytes+store+norepinephrine+by+vesicular+transport%5BTitle%5D)+AND+%22Arterioscler+Thromb+Vasc+Biol%22%5BJournal%5D)">Perivascular adipocytes store norepinephrine by vesicular transport.</a> Arterioscler Thromb Vasc Biol. 39 (2), 188-199 (2019).</li>
<li>Ya-Jing, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adipose+tissue-derived+cytokines%2C+CTRPs+as+biomarkers+and+therapeutic+targets+in+metabolism+and+the+cardiovascular+system%5BTitle%5D)+AND+%22Vessel+Plus%22%5BJournal%5D)">Adipose tissue-derived cytokines, CTRPs as biomarkers and therapeutic targets in metabolism and the cardiovascular system.</a> Vessel Plus. (1), 202-212 (2017).</li>
<li>Osol, G., Brekke, J. F., McElroyYaggy, K., Gokina, N. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myogenic+tone%2C+reactivity%2C+and+forced+dilatation%3A+A+threephase+model+of+in+vitro+arterial+myogenic+behavior%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Myogenic tone, reactivity, and forced dilatation: A threephase model of in vitro arterial myogenic behavior.</a> Am J Physiol Heart Circ Physiol. 283 (6), H2260-H2267 (2002).</li>
</ol>

<table><tbody><tr><td>Cloreto de acetilcolina</td><td>Sigma-Aldrich, Merck, Darmstadt, Alemanha</td><td>Resposta A6625</td><td></td></tr><tr><td>Prato de cultura de fundo preto</td><td>Tecnologia Myo dinamarquesa, Aarhus, Dinamarca</td><td>300412</td><td></td></tr><tr><td>CaCl2</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A501330</td><td></td></tr><tr><td>D-glicose</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A610219</td><td></td></tr><tr><td>Pinça Dumont</td><td>Tecnologia Myo dinamarquesa, Aarhus, Dinamarca</td><td>300413</td><td></td></tr><tr><td>Tesoura de dissecção Geuder</td><td>Tecnologia Myo dinamarquesa, Aarhus, Dinamarca</td><td>400431</td><td></td></tr><tr><td>Fio-guia</td><td>Tecnologia Myo dinamarquesa, Aarhus, Dinamarca</td><td>400447</td><td>Diâmetro 40 &micro; m</td></tr><tr><td>Kcl</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A100395</td><td></td></tr><tr><td>KH2PO4</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A100781</td><td></td></tr><tr><td>LabChart Professional versão 8.3&nbsp;</td><td>ADInstruments, Austrália</td><td>-</td><td></td></tr><tr><td>MgCl2&middot; 6H2O</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A100288</td><td></td></tr><tr><td>Sistema multi-miógrafo</td><td>Tecnologia Myo dinamarquesa, Aarhus, Dinamarca</td><td>620 milhões</td><td></td></tr><tr><td>NaCl</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A100241</td><td></td></tr><tr><td>NaHCO3</td><td>Sangon Biotech Co., Ltd., Xangai, China</td><td>A100865</td><td></td></tr><tr><td>Fenilefrina</td><td>Sigma-Aldrich, Merck, Darmstadt, Alemanha</td><td>Pág. 6126</td><td></td></tr></tbody></table>

microdissecção multinível e perfil funcional-estrutural de ramos arteriais renais humanos

Multilevel Microdissection and Functional-Structural Profiling of Human Renal Arterial Branches

No rim humano, a artéria interlobar corre entre as pirâmides renais ao lado de sua veia correspondente. Caracterizada por uma parede vascular relativamente espessa e um invólucro significativo de tecido adiposo, essa artéria requer uma dissecção particularmente cuidadosa. O tecido adiposo circundante deve ser removido meticulosamente para evitar a aplicação de força excessiva durante o procedimento de isolamento (Figura 1C).
A artéria arqueada está anatomicamente ...

Microdissecção Multinível e Perfil Funcional-Estrutural de Ramos Arteriais Renais Humanos

Renal vascular dysfunction plays a critical role in the pathogenesis of multiple clinical conditions, including acute kidney injury, renal ischemia, and ...

multilevel-microdissection-functional-structural-profiling-human

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O grau de disfunção vascular e contribuindo mecanismos fisiológicos podem ser avaliados em pacientes com doença renal crônica através da medição do fluxo-mediada dilatação da artéria braquial, a velocidade da onda de pulso da aorta, e expressão da proteína de célula endotelial vascular.

Avaliação da função vascular em pacientes com doença renal crônica

O rim adulto do peixe-zebra é um excelente sistema para os estudos de regeneração e de doenças renais. Um aspecto essencial dessa pesquisa é a avaliação da estrutura e função de néfrons. Este protocolo descreve várias metodologias que podem ser implementadas para avaliar a composição de néfrons túbulo e avaliar a reabsorção renal.

Análise de Composição Nephron e função no rim adulto Zebrafish

O uso de ressonância magnética de campo ultra-alto como forma não invasiva de obter informações fenotípicas de modelos de roedores para doença renal policística e monitorar intervenções é descrito. Em comparação com a abordagem histológica tradicional, as imagens de ressonância magnética podem ser adquiridas in vivo, permitindo o acompanhamento longitudinal.

Uso de ultra-alta MRI campo em pequeno roedor modelos de doença renal policística para<em&gt; In Vivo</em&gt; Fenotipagem and Drug Monitoring

Este protocolo descreve um método para isolar e cultivar rudimentos metanefricos de embriões de ratinho.

Dissecção e cultura do rim embrionário do rato

Engenharia de tecido renais construções oferecem uma solução para a escassez de órgãos e os efeitos deletérios da diálise. Aqui, descrevemos um protocolo para micro dissecar murino rins para isolamento dos segmentos córtico-medular. Esses segmentos são implantados livre de andaime celular construções, formando organoids renal.

Microdissection de segmentos de tecido Renal primária e incorporação com tecnologia de construção romance livre de andaime

Um modelo modificado de 2 rim 1 (2K1C) O modelo de rato Goldblatt foi desenvolvido usando tubos de poliuretano para iniciar a estenose da artéria renal, induzindo um aumento na expressão de renin e lesão renal. Aqui, descrevemos um procedimento detalhado de preparação e colocação da braçadeira na artéria renal para gerar um modelo de mouse 2K1C reprodutível e consistente.

Um modelo modificado de rato de dois rins um clipe de regulação de renin em estenose da artéria renal

Descrevemos um protocolo para microdisseção a laser de subsegmentos do rim humano, incluindo o glomerulus, túbulo proximal, membro ascendente espesso, coletor de duto e interstício. O RNA é então isolado dos compartimentos obtidos e o sequenciamento de RNA é realizado para determinar alterações na assinatura transcriômica dentro de cada subsegmento.

Aplicação de microdisseção a laser para descobrir transcrição regional em tecido renal humano

O protocolo descreve o isolamento, a cultura e o perfil das células endoteliais da artéria mesentérica humana. Além disso, é fornecido um método para preparar a artéria humana para transcrição espacial. Os ensaios proteômicos, transcriptomics e funcionais podem ser realizados em células isoladas. Este protocolo pode ser reaproveitado para qualquer artéria de médio ou grande porte.

Isolamento e Perfil das Células Endoteliais Mesentóricas Primárias Humanas no Nível Transcriptome

Aqui, apresentamos um protocolo utilizando microscopia de 2 fótons em ratos Wistar Fromter de Munique com glomérulos superficiais para quantificar os efeitos da obstrução ureteral prolongada na dinâmica e função glomerular.

Usando a microscopia de 2-fótons para quantificar os efeitos da obstrução ureteral unilateral crônica nos processos glomerulares

Aqui, apresentamos um protocolo detalhado para análise proteômica de todo o rim, túbulo cortical isolado e proteomas medulares. O estudo também compara proteomas regionais em um modelo de camundongo diabético e camundongos não diabéticos.

Análise proteômica comparativa de rins inteiros, medula e túbulos corticais na patogênese diabética da lesão renal em camundongos

Aprenda a isolar e avaliar funcionalmente ramos arteriais renais humanos usando microdisseção e miografia de arame. Este método permite análise vascular precisa em modelos humanos específicos, superando limitações de espécies animais e melhorando o desenvolvimento translacional de fármacos.

Microdissecção Multinível e Perfil Funcional-Estrutural de Ramos Arteriais Renais Humanos

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