Geração e Análise Transcriptômica de Célula Única de Organoides de Carcinoma Hepatocelular após Tratamento Medicamentoso

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08:18 min.

26 de maio de 2026

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Dan He^*¹ , Zhong Liang^*¹^,²^,³ , Cheng-Yang Zhang^*¹^,²^,³ , Jie-Shen Xian^*⁴ , Yuan-Xiang Yang⁵ , Yuan-Feng Gong³ , Hui Tang³ , Guang-Ding Zhou¹^,²^,³ , Chen-Yu Jiang¹^,²^,³ , Le Xing¹^,²^,³ , Dong-Mei Li¹^,²^,³ , Ying-Chen Hou¹^,²^,³ , Chen Yue¹^,²^,³ , Wei Cheng¹^,² , Yun-Qiang Tang³ , Ming Liu¹^,²^,³ , Xiao-Feng Zhang¹^,²^,³^,⁶

¹School of Basic Medical Science, Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Guangzhou Medical University, ³Affiliated Cancer Hospital and Institute, Guangzhou Medical University, ⁴The First Clinical School of Guangzhou Medical University, Guangzhou Medical University, ⁵Department of Obstetrics, Guangzhou Eighth People's Hospital, Guangzhou Medical University, ⁶Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University

^* These authors contributed equally

Este protocolo descreve um método simplificado para gerar organoides de carcinoma hepatocelular, aplicar tratamento medicamentoso e realizar sequenciamento de RNA unicelular antes e depois do tratamento para caracterizar alterações transcricionais associadas ao tratamento.