JOVE JOURNAL

Biology

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Journal (Biology)

Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

Gaëlle Letort², Philippe Mailly, Adel Al Jord³, Maria Almonacid

¹Center for Interdisciplinary Research in Biology (CIRB),Collège de France, CNRS, INSERM, Université PSL, ²Department of Developmental and Stem Cell Biology,Institut Pasteur, Université de Paris Cité, CNRS, ³Centre for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology

Journal (Biology)

Oocyte Collection, Preparation, and Microinjection in Mouse for Live Imaging Studies

Gaëlle Letort², Philippe Mailly, Adel Al Jord³, Maria Almonacid

¹Center for Interdisciplinary Research in Biology (CIRB),Collège de France, CNRS, INSERM, Université PSL, ²Department of Developmental and Stem Cell Biology,Institut Pasteur, Université de Paris Cité, CNRS, ³Centre for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology

Journal (Biology)

Live Cell Imaging and Analysis of Nuclear Dynamics in Mouse Oocytes

Gaëlle Letort², Philippe Mailly, Adel Al Jord³, Maria Almonacid

¹Center for Interdisciplinary Research in Biology (CIRB),Collège de France, CNRS, INSERM, Université PSL, ²Department of Developmental and Stem Cell Biology,Institut Pasteur, Université de Paris Cité, CNRS, ³Centre for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology

Journal (Biology)

Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence

Louai Zaidan*¹, Perla Geara*¹, Matthew J. Borok*¹, Léo Machado, Despoina Mademtzoglou, Philippos Mourikis, Frederic Relaix^2,3,4

¹Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, ²Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, ³EFS, IMRB, F-94010 Creteil, France, ⁴AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France

Journal (Biology)

Harvesting and Digesting Mouse Skeletal Muscle for 2D Culture of Niche and Stem Cells

Louai Zaidan*¹, Perla Geara*¹, Matthew J. Borok*¹, Léo Machado, Despoina Mademtzoglou, Philippos Mourikis, Frederic Relaix^2,3,4

¹Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, ²Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, ³EFS, IMRB, F-94010 Creteil, France, ⁴AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France

Journal (Biology)

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Michael Finnegan, Jeanne Hamet, Erick Desmarais, Stéphanie Bedhomme

¹CEFE,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France, ²ISEM,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France

Journal (Biology)

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Antonia Hauth, Rafael Galupa, Nicolas Servant, Laura Villacorta, Kai Hauschulz, Joke Gerarda van Bemmel, Agnese Loda, Edith Heard⁵

¹EMBL: European Molecular Biology Laboratory, ²Institut Curie, ³Agilent Technologies, ⁴Genmab BV, ⁵Collège de France

Journal (Biology)

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Aline Stedman³, Antonin Levy⁴, Philippe J. Sansonetti^2,5, Giulia Nigro⁶

¹Molecular Microbial Pathogenesis Unit,Institut Pasteur, ²Chaire de Microbiologie et Maladies Infectieuses,Collège de France, ³Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit,Sorbonne Université, CNRS UMR7622, INSERM U1156, ⁴Molecular Radiotherapy, INSERM U1030, Gustave Roussy,Université Paris-Saclay, ⁵The Center for Microbes, Development and Health,Institut Pasteur Shanghai and Chinese Academy of Sciences, ⁶Microenvironment and Immunity Unit,Institut Pasteur

Journal (Biology)

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

Josselin Rigaill*^1,2, Estelle Audoux*¹, Killian Rodriguez, Aurélien Peyron, Philippe Berthelot³, Jérôme Josse⁵, Frédéric Laurent⁵, Robin Caire, Paul O. Verhoeven²

¹CIRI, Centre International de Recherche en Infectiologie, GIMAP team,University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, ²Department of Infectious Agents and Hygiene,University Hospital of St-Etienne, St-Etienne, France, ³Department of Infectious Diseases,University Hospital of St-Etienne, St-Etienne, France, ⁴CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team,University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, ⁵Department of Bacteriology, Institute for Infectious Agents,Hospices Civils de Lyon, Lyon, France

Journal (Biology)

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Jennifer Jager, Mélanie Gaudfrin, Jérôme Gilleron, Mireille Cormont, Jean-François Tanti

¹Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France

Journal (Biology)

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles (“Nanoblades”)

Philippe E. Mangeot, Laura Guiguettaz, Thibault J. M. Sohier, Emiliano P. Ricci

¹LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, ²CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

Journal (Biology)

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Hugo Lachuer^2,3, Pallavi Mathur^2,3, Kevin Bleakley, Kristine Schauer^2,3

¹Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group,Institut Curie, 75005 Paris, France, ²PSL Research University, Paris, France, ³Sorbonne Université, Paris, France, ⁴INRIA, Université Paris-Sud, PSL

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