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Following the Dynamics of Structural Variants in Experimentally Evolved Populations
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Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

DOI:
10.3791/64709-v

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04:52 min

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February 03, 2023

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, , ,
1CEFE,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France, 2ISEM,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France

Chapters

  • 00:05Introduction
  • 00:52PCR Optimization
  • 01:57Parallel Capillary Electrophoresis and Calibration
  • 03:18Results: Mutant Allele Expression
  • 04:21Conclusion

Summary

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We developed a cost-effective method to follow non-single nucleotide polymorphism allele dynamics that can easily be adapted to experimental evolution frozen archives. A triplet PCR technique was coupled with automated parallel capillary electrophoresis to quantify the relative frequency of an insertion allele over the course of experimental evolution.

Tags

Keywords: Structural VariantsExperimental EvolutionCapillary ElectrophoresisIS10 InsertionMutS GenePCRDNA QuantificationCalibration CurveParallel Capillary Electrophoresis

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