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Методы Именно локализованного передачи ячеек или ДНК в начале постимплантационных эмбрионов мыши
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
JoVE Journal
Биология развития
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JoVE Journal Биология развития
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Методы Именно локализованного передачи ячеек или ДНК в начале постимплантационных эмбрионов мыши

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09:04 min

December 25, 2015

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09:04 min
December 25, 2015

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We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

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