Chapter 16
Analyzing Gene Expression and Function
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by…
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a…
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first…
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum…
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more…
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied…
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced…
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at…
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes…
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a…
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell…
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively…
Brain development is a complex process, which is controlled in a temporo-spatial manner by gradients of morphogens and different transcriptional…
Toxoplasma gondii, the causative agent of toxoplasmosis, is an obligate intracellular protozoan pathogen. The parasite invades and replicates within…
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed for…