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第15章
What is Genetic Engineering?
Genetic engineering is the process of modifying an organism’s DNA to introduce new, desirable traits. Many organisms, from bacteria to plants…
Antibiotic Selection
Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance…
重组DNA
Scientists create recombinant DNA by combining DNA from different sources—often, other species—in the laboratory. DNA cloning allows…
转基因生物
Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The…
成体干细胞
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell…
胚胎干细胞
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous…
诱导多能干细胞
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific…
体外致突变性
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by…
DNA分离
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells,…
基因治疗
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a…
生殖性克隆
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced…
基因编辑技术
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at…
互补DNA
Only genes that are transcribed into messenger RNA (mRNA) are active, or expressed. Scientists can, therefore, extract the mRNA from cells to study…
PCR
The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce…
基因组学
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried…
另类文化的人多能干细胞的生产,维护和遗传分析
Human pluripotent stem cells (hPSCs) hold great promise for regenerative medicine and biopharmaceutical applications. Currently, optimal culture and…
有针对性的下一代测序和生物信息学管道评价宪政疾病的遗传决定因素
Next-generation sequencing (NGS) is quickly revolutionizing how research into the genetic determinants of constitutional disease is performed. The…
多器官芯片 - 一种微流体平台的长期多组织共培养
The ever growing amount of new substances released onto the market and the limited predictability of current in vitro test systems has led to a high…
哺乳动物系统 CRISPR 指南 RNA 克隆
The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative…
CAPRRESI:通过质粒恢复和限制酶位点插入的嵌合体装配
Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI). CAPRRESI benefits from many strengths of the…
基于角蛋白纳米纤维生物医学工程合成
Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers.…
协议的射频评估与互动金纳米粒子和生物系统的非侵入性癌症热疗治疗
Cancer therapies which are less toxic and invasive than their existing counterparts are highly desirable. The use of RF electric-fields that…

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