JoVE Lab Manual
Lab Bio
Lab Bio
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Trasformazione batterica
Trasformazione batterica
Lab Prep
- Bacterial Transformation
- First, ensure that each student or student group has a filled ice bucket.
- Then, set up a tube rack for each work station.
- Into the tube racks, place two empty, sterile 1.5 mL tubes, and then two one mL aliquots of SOC media.
- Then, place the plasmid tube into the ice bucket and add two tubes of competent E. coli cells, allowing them to thaw if frozen.
- Set out two LB agar plates per group and two LB agar ampicillin plates per group.
- Ensure the students have the appropriate pipettes.
- And finally, set a water bath to 42 °C and a temperature-controlled shaker to 37 °C.
