JoVE Lab Manual
Lab: Chemistry
Lab: Chemistry
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Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA
Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.
All stock solutions in 50 mL solvent | |||
Dye | Stock concentration | Mass | Solvent |
β-carotene | 1 x 10-3 (1 mM) | 0.027 g | Hexane |
Indigo | 1 x 10-3 (1 mM) | 0.013 g | DMF |
All test solutions diluted to 50 mL | |||
Dye | Test concentration | Stock volume | Solvent |
β-carotene | 3 x 10-6 (3 µM) | 150 µL | Hexane |
Indigo | 3 x 10-6 (3 µM) | 150 µL | DMF |
All solutions diluted to 50 mL with hexane | ||
Dye | Concentration | Stock volume |
β-carotene | 1.9 x 10-6 (1.9 µM) | 95 µL |
β-carotene | 3.7 x 10-6 (3.7 µM) | 185 µL |
β-carotene | 7.5 x 10-6 (7.5 µM) | 375 µL |
β-carotene | 11 x 10-6 (11 µM) | 550 µL |
β-carotene | 15 x 10-6 (15 µM) | 750 µL |