Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Click here for the English version

Biology

Het maken van Gynogenetic Diploïde zebravis in het begin van Pressure

Published: June 30, 2009 doi: 10.3791/1396

Materials

Name Company Catalog Number Comments
Tricaine Sigma-Aldrich
Watch glass
French Press
Pressure cylinder
Instant ocean
Pasteur pipettes Cut off the narrow end, fire polish
UV cross-linker
microcapillaries

DOWNLOAD MATERIALS LIST

References

  1. Streisinger, G. Segregation analyses and gene-centromere distances in zebrafish. Genetics. 112 (2), 311-311 (1986).
  2. Streisinger, G. Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature. 291 (5813), 293-293 (1981).
  3. Westerfield, M. The Zebrafish Book: A guide for the laboratory use of zebrafish (Danio rerio). , 4th ed, University of Oregon Press. Eugene, Oregon. (2000).
  4. Johnson, S. L., Africa, D., Horne, S., Postlethwait, J. H. Half-tetrad analysis in zebrafish: mapping the ros mutation and the centromere of linkage group I. Genetics. 139 (4), 1727-1727 (1995).
  5. Beattie, C. E., Raible, D. W., Henion, P. D., Eisen, J. S. Early pressure screens. Methods Cell Biol. 237 (71), 2575-25 (1999).
  6. Johnson, S. L. Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics. 142 (4), 1277-1277 (1996).
  7. Trede, N. S. Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen. Dev Dyn. 237 (9), 2575-2575 (1999).
Het maken van Gynogenetic Diploïde zebravis in het begin van Pressure
Play Video
PDF DOI DOWNLOAD MATERIALS LIST

Cite this Article

Walker, C., Walsh, G. S., Moens, C.More

Walker, C., Walsh, G. S., Moens, C. Making Gynogenetic Diploid Zebrafish by Early Pressure. J. Vis. Exp. (28), e1396, doi:10.3791/1396 (2009).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter