Irreversible Electroporation: A Technique for Ablating Pancreatic Tumor in Mouse

Abstract

Source: Shankara Narayanan, J. S., et al. A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation. J. Vis. Exp. (2018).

This video describes the procedure for performing irreversible electroporation (IRE) for non-thermal ablation of pancreatic tumors in mice. Both subcutaneous (SQ) and orthotopic tumors can be successfully treated with IRE in a highly controlled setting, facilitating various studies post-procedure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board.

1. IRE of SQ Tumors

1. Prepare the materials required: square wave electroporator, safety foot switch, electrodes (needle array versus tweezer-trode), and adaptors. Keep sterile sutures (non-absorbable 4-0), alcohol swabs, hair clippers, depilatory cream, eye lubricant, cotton gauze, buprenorphine, and anesthetics also nearby.
2. Sterilize the tweezer or needle array electrodes using gas sterilization. Autoclaving is not recommended. Use a glass bead sterilizer to sterilize ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
ECM 830 square wave electroporator	Harvard Apparatus	BTX # 45-0002 ( 58018-004 )
2 needle array electrode	Harvard Apparatus	45-0167
Safety foot switch	Harvard Apparatus	45-0211
Platinum Tweezer-trode	Harvard Apparatus	45-0486
Isoflurane	Sigma-Aldrich, Inc.	792632
Lacrilube	Fisher Scientific	19090646
Buprenorphine	Fisher Scientific	NC1292810
D-luciferin	Perkin Elmer	122799
IVIS Spectrum In Vivo Imaging System	Perkin Elmer	124262
Mouse strain C57BL/6J	The Jackson Laboratory	000664/Black 6
BD Precisionglide syringe needles	Sigma-Aldrich, Inc.	Z192406
Alcohol Swab(70% isopropyl alcohol )	BD	326895
Disposable Scalpels, Sterile	VWR	21909
Cotton Tipped Applicators	VWR	89198
Suture Needle, 45 cm, Size 6-0	Harvard Apparatus	72-3308
Suture Needle, 45 cm, Size 4-0	Harvard Apparatus	72-3314
Povidone-iodine 10%	BD	29900-404
Disposable Warming Pad	KENT SCIENTIFIC CORP	TP-3E
Mouse Hair Clipper	KENT SCIENTIFIC CORP	CL8787
Surgical Drape	Harvard Apparatus	59-7421