Bioluminescent Orthotopic Pancreatic Cancer Mouse Model: A Non-invasive Technique to Monitor Cancer Progression in Mice

Abstract

Source: Chai, M. G., et al. Bioluminescent Orthotopic Model of Pancreatic Cancer Progression. J. Vis. Exp. (2013).

This video demonstrates an orthotopic model of pancreatic cancer that utilizes Matrigel for localized cell delivery and in vivo bioluminescence imaging for non-invasive monitoring of tumor progression. The orthotopic model is suited to both syngeneic and xenograft models and could be used in pre-clinical trials to investigate the impact of novel anti-cancer therapeutics on the growth of the primary pancreatic tumor and metastasis.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Transducing Pancreatic Cancer Cell Lines

1. Transduce pancreatic cancer cells to express luciferase as previously described. Panc-1 and Capan-1 pancreatic cancer cell lines transduced with firefly luciferase are used here.
   Note: Renilla luciferase or bacterial luciferase may also be used.

2. Pancreatic Cancer Cell Preparation

1. Culture transduced pancreatic cancer cells until 70% confluent.
2. Lift the....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Clean Bench coat
Heating pad			Set to 37 °C
Ivis Lumina ll Bioluminescent imager	Caliper		Alternative bioluminescent imaging systems include In vivo F PRO (Carestream) and Photon Imager (Biospace Lab)
Dissecting scissors
Iris forceps (serrated)
Needle holder
27 G 0.3 ml insulin syringe	Terumo	T35525M2913