संस्कृति दौरान स्थलाकृति बदलने की क्षमता के साथ सेल संस्कृति substrates के विकास के लिए एक विधि का वर्णन है. विधि स्मार्ट आकार स्मृति पॉलिमर है कि एक स्थायी आकार को याद करने की क्षमता है के रूप में जाना जाता है सामग्री का उपयोग करता है. इस अवधारणा को सामग्री और अनुप्रयोगों की एक विस्तृत श्रृंखला के लिए अनुकूलनीय है.
Shape memory polymers (SMPs) are a class of “smart” materials that have the ability to change from a fixed, temporary shape to a pre-determined permanent shape upon the application of a stimulus such as heat1-5. In a typical shape memory cycle, the SMP is first deformed at an elevated temperature that is higher than its transition temperature, Ttrans [either the melting temperature (Tm) or the glass transition temperature (Tg)]. The deformation is elastic in nature and mainly leads to a reduction in conformational entropy of the constituent network chains (following the rubber elasticity theory). The deformed SMP is then cooled to a temperature below its Ttrans while maintaining the external strain or stress constant. During cooling, the material transitions to a more rigid state (semi-crystalline or glassy), which kinetically traps or “freezes” the material in this low-entropy state leading to macroscopic shape fixing. Shape recovery is triggered by continuously heating the material through Ttrans under a stress-free (unconstrained) condition. By allowing the network chains (with regained mobility) to relax to their thermodynamically favored, maximal-entropy state, the material changes from the temporary shape to the permanent shape.
Cells are capable of surveying the mechanical properties of their surrounding environment6. The mechanisms through which mechanical interactions between cells and their physical environment control cell behavior are areas of active research. Substrates of defined topography have emerged as powerful tools in the investigation of these mechanisms. Mesoscale, microscale, and nanoscale patterns of substrate topography have been shown to direct cell alignment, cell adhesion, and cell traction forces7-14. These findings have underscored the potential for substrate topography to control and assay the mechanical interactions between cells and their physical environment during cell culture, but the substrates used to date have generally been passive and could not be programmed to change significantly during culture. This physical stasis has limited the potential of topographic substrates to control cells in culture.
Here, active cell culture (ACC) SMP substrates are introduced that employ surface shape memory to provide programmed control of substrate topography and deformation. These substrates demonstrate the ability to transition from a temporary grooved topography to a second, nearly flat memorized topography. This change in topography can be used to control cell behavior under standard cell culture conditions.
NOA63 के टी जी आसानी से इलाज के तापमान के माध्यम से नियंत्रित किया जा सकता है . हम इस प्रयोग एसएमपी substrates कि एक सेल संगत रेंज में चालू किया जा सकता है उत्पन्न. NOA63 पानी जो सूखी टी जी को कम करती है द्वारा plasti…
The authors have nothing to disclose.
लेखकों के लिए तकनीकी सहायता के लिए एसीसी सब्सट्रेट तैयारी के साथ केली ए बर्क धन्यवाद देना चाहूंगा. 10.1016/j.biomaterials.2010.12.006, कॉपीराइट Elsevier (2011): बायोमैटिरियल्स में प्रकाशित लेख, डेविस के.ए., एट अल, आकार स्मृति बहुलक substrates, बायोमैटिरियल्स, doi पर गतिशील सेल व्यवहार के आधार पर. इस सामग्री को अनुदान DMR 0907578 – सं के तहत NSF द्वारा समर्थित काम पर आधारित है.
Name of the reagent or instrument | Company | Catalogue number | Comments (optional) |
NOA63 | Norland Products Inc. | NOA63 | Lot number 111 |
Dogbone Punch | TestResource, Inc. Shakopee, MN | Scaled-down Type IV dogbone (ASTM D638-03) | |
Benchtop Hydraulic Press | Carver | 3851 | |
C3H10T1/2 Mouse Embryonic Fibroblasts | ATCC | CCL-226 | |
Biological Safety Cabinet | Thermo Fisher | 1357 | |
UV Lamp | Spectroline | SB-100PC | |
Dynamic Mechanical Analyzer (DMA) | TA Instruments, Inc. | Q800 | |
Inverted Fluorescence Microscope | Leica | Leica DMI 4000B | |
Confocal Laser Scanning Microscope | Zeiss | LSM 710 | 20x/0.8 NA air or a 40x/1.30 NA oil objective |