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Immunology and Infection

Diagnóstico da tuberculose pulmonar com o teste Xpert MTB / RIF

Published: April 9, 2012 doi: 10.3791/3547

Materials

Name Company Catalog Number Comments
Xpert MTB/RIF test Cepheid GXMTB/RIF-10
Transfer pipettes Sarstedt Ltd 86.1172.001
Conical tubes, 15 ml, 120 x 17 mm, PS Sarstedt Ltd 62.553.542

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References

  1. Sudre, P., Dam, ten, Chan, G., C,, Kochi, A. Tuberculosis in the present time: a global overview of the tuberculosis situation. World Health Organisation Document WHO/TUB/91. 158, 1-47 (1991).
  2. World Health Organisation. Global tuberculosis control: epidemiology, strategy, financing. WHO report 2010. , (2010).
  3. The global plan to stop TB, 2006-2015. , World Health Organization. Geneva. (2006).
  4. Morris, S., et al. Molecular mechanisms of multiple drug resistance in clinical isolates of Mycobacterium tuberculosis. J. Infect. Dis. 171, 954-960 (1995).
  5. Traore, H., Fissette, K., Bastian, I., Devleeschouwer, M., Portaels, F. Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as aninitial indicator of multidrug resistance. Int. J. Tuberc. Lung Dis. 4, 481-484 (2000).
  6. Helb, D., et al. Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand Near-Patient Technology. J. Clin. Microbiol. 48, 229-237 (2010).
  7. Blakemore, R., et al. Evaluation of the Analytical Performance of the Xpert MTB/RIF. Assay. J. Clin. Microbiol. 48, 2495-2501 (2010).
  8. Telenti, A., et al. Detection of rifampicin-resistance in Mycobacterium tuberculosis. Lancet. 341, 647-650 (1993).
  9. GeneXpert Dx System Operator Manual. , Cepheid Inc. Sunnyvale, CA, USA. (2011).
  10. Foundation for new innovative diagnostics [Internet]. , Geneva, Switzerland. Available from: http://www.finddiagnostics.org/about/what_we_do/successes/find-negotiated-prices/xpert_mtb_rif.html (2011).
  11. International Standards for Tuberculosis Care (ISTC). , Tuberculosis Coalition for Technical Assistance. The Hague. (2009).
  12. Boehme, C. C., et al. Rapid Molecular Detection of Tuberculosis and Rifampin Resistance. N. Engl. J. Med. 363, 1005-1015 (2010).
  13. Marlowe, E. M., et al. Evaluation of the Cepheid Xpert MTB/RIF assay for the Direct Detection of Mycobacterium tuberculosis Complex from Respiratory Specimens. J. Clin. Microbiol. 49, 1621-1623 (2011).
  14. El-Hajj, H. H., Marras, S. A. M., Tyagi, S., Kramer, F. R., Alland, D. Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons. J. Clin. Microbiol. 39, 4131-4137 (2001).
  15. Hillemann, D., Ruesch-Gerdes, S., Boehme, C., Richter, E. Rapid molecular detection of extrapulmonary tuberculosis by automated GeneXpert MTB/RIF system. J. Clin. Microbiol. 49, 1202-1205 (2011).
Diagnóstico da tuberculose pulmonar com o teste Xpert MTB / RIF
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Cite this Article

Bodmer, T., Ströhle, A.More

Bodmer, T., Ströhle, A. Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test. J. Vis. Exp. (62), e3547, doi:10.3791/3547 (2012).

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