Materials
Name | Company | Catalog Number | Comments |
22x22 mm microscope coverslips | Fisher | 12-544-10 | |
18x18 mm microscope coverslips | Fisher | 12-553-402 | |
Acetic acid | Fisher | A491-212 | |
Methanol | Fisher | A412-4 | |
Propionic acid | Sigma-Aldrich | 402907 | |
Dremel 200 rotary tool with a Flex-Shaft attachment | Rand | 3" Multi-purpose Mini Bench Grinder (with rate limiter) | |
Specially designed 200 μl plastic tips (beaded plastic edges) for Dremel tool | Pipetman | F171300 | Cut and heat fat end of pipet tips |
Tin coated rapid vise | Avenger Gold Toolmaker | MTC-200-1 | Precision ground square and parallel to within 0.00025 |
Torque wrench | Craftsman | 44593 | |
ThermoBrite Slide Denaturation/Hybridization System | Abbott Molecular | 30-144110 | |
25 mm barrier slides | Abbott Molecular | XT108-SL | |
25 mm coverslips | Abbott Molecular | XT122-90X | |
Xmatrx Automated Slide Staining System | Abbott Molecular | 08L46-001 | |
MZ6 Leica stereomicroscope | Leica | VA-OM-E194-354 | A different stereomicroscope can be used |
Olympus CX41 Phase Microscope | Olympus | CX41RF-5 | A different phase microscope can be used |
ACCORD PLUS Automated Scanning System | BioView (USA) | BV-5000-ACCP | |
10x PBS | Invitrogen | P5493 | |
10% NBF (neutral buffered formalin) | Sigma-Aldrich | HT501128 | |
99% formamide | Fisher Scientific | BP227500 | |
Dextran sulfate sodium salt | Sigma | D8906 | |
20x SSC buffer | Invitrogen | AM9765 | |
Sodium phosphate | Sigma-Aldrich | S3264 | |
50x Denhardt's solution | Sigma-Aldrich | D2532 | |
Sodium azide | Sigma-Aldrich | S2002 | |
1 mM YOYO-1 iodide (491/509) solution in DMSO | Invitrogen | Y3601 | |
ProLong Gold antifade reagent | Invitrogen | P36930 | |
dATP, dCTP, dGTP, dTTP | Fermentas | R0141, R0151, R0161, R0171 | |
Cy3-dUTP, Cy5-dUTP | GE Healthcare | PA53022, PA55022 | |
BSA | Sigma | A3294 | |
DNA polymerase I | Fermentas | EP0041 | |
DNase I | Fermentas | EN0521 |
References
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