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Published: February 20, 2013 doi: 10.3791/4434


Name Company Catalog Number Comments
Finquel (Tricaine methanesulfonate) Argent Chemical Laboratories MS-222 Larvae anesthesia
Phenol Red Solution Sigma-Aldrich P0290-100 ml 0.5% in DPBS, cell-culture tested
Mineral oil, light, white (high purity) Amresco J217-500 ml For needle backfill and volume testing
TxRed-Dextran, 10,000 MW, lysine fixable Invitrogen/ Molecular Probes D-1863 1% suspension in 1x PBS/phenol red
FM4-64FX Invitrogen/ Molecular Probes F34653 5 mM stock in water
Methylcellulose MP Biochemicals 0215549590
Borosilicate glass capillaries Drummond Scientific 3-000-203-G/X OD 1.14 mm, ID 0.53 mm, 3.5 in length
Plastic form for mold making Adaptive Science Tools TU-1
Nanoject II microinjection unit Drummond Scientific 3-000-204
Flaming Brown Micropipette Puller, 3.0 mm wide-trough filament Sutter Instrument Co. P-97, FT330B Needle fabrication
Student Dumont #5 forceps Fine Science Tools 91150-20 0.1 x 0.06 mm, needle clipping
Eyepiece Graticle, 5 mm - 100 divisions Leica 10394771 Needle clipping
Microforge Narishige MF-900 Needle clipping and fire-polishing
Tuberculin SlipTip syringe needle Becton Dickinson 309626 1 ml, 25G 5/8-needle
Fluoresbrite YG Microspheres (2.0 μm) Polysciences, Inc. 18338 Intestinal motility analysis
Dissecting Microscope Leica S6E Gavage procedure
Fluorescence Stereomicroscope Leica M205C Dextran barrier assay
Confocal microscope Zeiss LSM510 Imaging of dextran in circulation



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Cocchiaro, J. L., Rawls, J. F.More

Cocchiaro, J. L., Rawls, J. F. Microgavage of Zebrafish Larvae. J. Vis. Exp. (72), e4434, doi:10.3791/4434 (2013).

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