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En orthotopic Blærekreft Modell for Gene Levering Studies

doi: 10.3791/50181 Published: December 1, 2013


Name Company Catalog Number Comments

Name of reagent


Catalog number


6-8 week old female mice

Jackson Laboratories

Strain Name: C57BL/6J

Stock Number: 000664




Obtained through Fisher




Obtained through Fisher





T25 flasks*

Corning Costar

Corning No.:3056

Fisher: 07-200-63

Obtained through Fisher

MB49 cells



Obtained from Dr. Boehle (see reference11)

Puralube Vet Ointment*


Henry Schein Company


Fisher: NC9676869

Obtained through Fisher

Depilatory cream: Veet

local pharmacy


K-Y Jelly

local pharmacy


Exel International

Exel International


Fisher: 14-841-21

Obtained through Fisher



NDC 66794-011-25

Obtained though hospital pharmacy

1 ml slip tip TB syringes

Becton Dickinson




Gold Biotechnologies




1000; Request large scale amplification and CsCl purification for in vivo use

Infectious agent that requires BSL2 containment

Steady-Glo Luciferase Assay System


E2510 (10 ml), E2520 (100 ml), or E2550 (10 x 100 ml)

*available through multiple vendors


Anesthesia system

E-Z Systems, Euthanex Corporation

Anesthesia system: EZ7000

5-port mouse rebreathing device: EZ109

Obtained through Fisher

Xenogen IVIS 200

Caliper Life Sciences


FLUOstar Optima

BMG Labtech




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En orthotopic Blærekreft Modell for Gene Levering Studies
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Cite this Article

Kasman, L., Voelkel-Johnson, C. An Orthotopic Bladder Cancer Model for Gene Delivery Studies. J. Vis. Exp. (82), e50181, doi:10.3791/50181 (2013).More

Kasman, L., Voelkel-Johnson, C. An Orthotopic Bladder Cancer Model for Gene Delivery Studies. J. Vis. Exp. (82), e50181, doi:10.3791/50181 (2013).

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