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December 2012: This Month in JoVE

Published: December 3, 2012 doi: 10.3791/5047
1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production


Here are some highlights from the December 2012 Issue of Journal of Visualized Experiments (JoVE).


Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with Loss of Peritoneal Integrity

Bruce A. Lessey1, H. Lee Higdon III1, Sara E. Miller2, Thomas A. Price3
1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Greenville Hospital System, 2Department of Pathology, Duke University Health System, 3Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Duke University

Loss of peritoneal integrity provides a new paradigm to understand and treat chronic pelvic pain in women with mild forms of endometriosis and can be easily detected using intraoperative instillation of dye at the time of laparoscopy.

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)

Samir Mujagić, Simon Michael Würth, Sven Hellbach, Volker Dürr
Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University

In this protocol we show how to condition harnessed honey bees to tactile stimuli and introduce a 2D motion capture technique for analyzing the kinematics of fine-scale antennal sampling pattern.

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica

Jeffrey M. McManus1, Hui Lu1, Hillel J. Chiel1, 2, 3
1Department of Biology, Case Western Reserve University , 2Department of Neurosciences, Case Western Reserve University , 3Department of Biomedical Engineering, Case Western Reserve University

We describe a technique to extracellularly record and stimulate from nerves, muscles, and individual identified neurons in vitro while eliciting and observing different types of feeding behaviors in the feeding apparatus of Aplysia.

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

Asad Raza, Chien-Chi Lin
Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis

The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Leslie Smith, Mathew Thayer
Department of Biochemistry and Molecular Biology, Knight Cancer Institute, Oregon Health & Science University

A quantitative method for the analysis of chromosome replication timing is described. The method utilizes BrdU incorporation in combination with fluorescent in situ hybridization (FISH) to assess replication timing of mammalian chromosomes. This technique allows for the direct comparison of rearranged and un-rearranged chromosomes within the same cell.

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Nalini Ramarao, Christina Nielsen-Leroux, Didier Lereclus
INRA, Micalis UMR1319, France

Oral and intra haemocolic infection of larvae of the greater wax moth Galleria mellonella is described. This insect can be used to study virulence factors of entomopathogenic as well as mammalian opportunistic bacteria. Rearing of the insects, methods of infection and examples of in vivo analysis are described.


No conflicts of interest declared.

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Chao, W., Kolski-Andreaco, A.More

Chao, W., Kolski-Andreaco, A. December 2012: This Month in JoVE. J. Vis. Exp. (70), e5047, doi:10.3791/5047 (2012).

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