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JoVE Journal Bioengineering
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Bioengineering

멀티 스펙트럼 이미징 유동 세포 계측법과 세포의 현지화 및 탄소 나노 튜브의 상대 정량

Published: December 12, 2013 doi: 10.3791/50566
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*1, *2, 3, 1, 1, 3, 1
1Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, 3Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire
* These authors contributed equally

Materials

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References

  1. Kostarelos, K., Bianco, A., Prato, M. Promises, facts and challenges for carbon nanotubes in imaging and therapeutics. Nat. Nano. 4, 627-633 (2009).
  2. De La Zerda, A., et al. Carbon nanotubes as photoacoustic molecular imaging agents in living mice. Nat. Nano. 3, 557-562 (2008).
  3. Zavaleta, C., et al. Noninvasive Raman Spectroscopy in Living Mice for Evaluation of Tumor Targeting with Carbon Nanotubes. Nano Lett. 8, 2800-2805 (2008).
  4. Tong, L., et al. Label-free imaging of semiconducting and metallic carbon nanotubes in cells and mice using transient absorption microscopy. Nat. Nano. 7, 56-61 (2012).
  5. Welsher, K., et al. A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. Nat. Nano. 4, 773-780 (2009).
  6. Jin, H., Heller, D. A., Sharma, R., Strano, M. S. Size dependent cellular uptake and expusion of single-wall carbon nanotubes: single particle tracking and a generic uptake model for nanoparticles. ACS Nano. 3, 149-158 (2009).
  7. Al-Jamal, K. T., et al. Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging. Nanoscale. 3, 2627-2635 (2011).
  8. Reuel, N. F., Dupont, A., Thouvenin, O., Lamb, D. C., Strano, M. S. Three-Dimensional Tracking of Carbon Nanotubes within Living Cells. ACS Nano. 6, 5420-5428 (2012).
  9. Phanse, Y., Ramer-Tait, A. E., Friend, S. L., Carrillo-Conde, B., Lueth, P., Oster, C. J., et al. Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry. J. Vis. Exp. (64), e3884 (2012).
  10. Qian, F., Montgomery, R. R. Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood. J. Vis. Exp. (62), e3741 (2012).
  11. Marangon, I., et al. Intercellular Carbon Nanotube Translocation Assessed by Flow Cytometry Imaging. Nano Lett. 12, 4830-4837 (2012).
  12. Lacerda, L., et al. Translocation mechanisms of chemically functionalised carbon nanotubes across plasma membranes. Biomaterials. 33, 3334-3343 (2012).
  13. Al-Jamal, K. T., Kostarelos, K. Methods in Molecular Biology. Balasubramanian, K., Burghard, M. 625, Humana Press. 123-124 (2010).
  14. Zhou, F., et al. New Insights of Transmembranal Mechanism and Subcellular Localization of Noncovalently Modified Single-Walled Carbon Nanotubes. Nano Lett. 10, 1677-1681 (2010).
멀티 스펙트럼 이미징 유동 세포 계측법과 세포의 현지화 및 탄소 나노 튜브의 상대 정량
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Cite this Article

Marangon, I., Boggetto, N.,… More

Marangon, I., Boggetto, N., Ménard-Moyon, C., Luciani, N., Wilhelm, C., Bianco, A., Gazeau, F. Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry. J. Vis. Exp. (82), e50566, doi:10.3791/50566 (2013).

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