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Medicine

रोधगलन के माउस मॉडल में एमआरआई और पीईटी

Published: December 19, 2013 doi: 10.3791/50806
Automatic Translation
1, 2, 1, 1, 1,3, 2
1Wolfson Brain Imaging Centre, Department of Clinical Neurosciences, Unversity of Cambridge, 2Department of Medicine, University of Cambridge, 3Behavioural and Clinical Neurosciences Institute, University of Cambridge

Abstract

रोधगलन पश्चिमी दुनिया में मृत्यु के प्रमुख कारणों में से एक है. मानव हृदय के लिए माउस को दिल की समानता यह उपन्यास चिकित्सीय रणनीतियों के परीक्षण के लिए एक आदर्श मॉडल बना दिया है.

विवो चुंबकीय अनुनाद इमेजिंग (एमआरआई) सही कार्यात्मक मूल्यांकन के लिए इस्तेमाल किया जा सकता है, जो स्पष्ट संरचनात्मक विस्तार, साथ noninvasively दिल की उत्कृष्ट विचारों देता है. इसके विपरीत एजेंटों ऊतक व्यवहार्यता की बुनियादी उपायों प्रदान कर सकते हैं, लेकिन इन अविशिष्ट हैं. पोजीट्रान एमिशन टोमोग्राफी (पीईटी) आणविक इमेजिंग के लिए अत्यधिक विशिष्ट है, लेकिन एमआरआई की संरचनात्मक विस्तार का अभाव है कि एक पूरक तकनीक है. एक साथ उपयोग किया है, इन तकनीकों के इलाज के बाद बीमारी और वसूली में दिल के आकलन के लिए एक संवेदनशील, विशिष्ट और मात्रात्मक उपकरण प्रदान करते हैं.

इस पत्र में हम इन तरीकों तीव्र रोधगलन के माउस मॉडल में किया जाता है की व्याख्या कैसे. प्रक्रियाओं घंटे वर्णितअरे ख्यात सुरक्षात्मक दवा उपचार के मूल्यांकन के लिए डिजाइन किए गए थे. हम देर gadolinium वृद्धि के साथ सिस्टोलिक समारोह और रोधगलितांश आकार को मापने के लिए एमआरआई का इस्तेमाल किया, और पीईटी fluorodeoxyglucose (FDG) के साथ infarcted क्षेत्र में चयापचय समारोह का आकलन करने के लिए. कागज ऐसे टुकड़ा नियोजन, सही gating, दवा वितरण, छवियों का विभाजन, और बहुविध coregistration के रूप में व्यावहारिक पहलुओं पर केंद्रित है. यहाँ प्रस्तुत तरीकों अच्छा repeatability और एक उच्च throughput बनाए रखने सटीकता प्राप्त.

Materials

Name Company Catalog Number Comments
Gadovist Bayer Schering Pharma PL 00010/0535
FDG IBA Molecular
Equipment
Bruker BioSpec 47/40 Bruker
Bruker mouse bed Bruker This bed includes tubing for anesthesia delivery and scavenging.
12 cm diameter birdcage transmitter Bruker T5346
2 cm diameter surface coil receiver Bruker T7027
Red Dot Neonatal monitoring electrodes 3M P/N: 2330
Monitoring equipment SA Systems The monitoring kit includes respiratory pillow and rectal probe for temperature measurements.
Anesthesia equipment General Anesthetic Services
Induction box Vet Tech Solutions LTD
Cambridge split-magnet PET/MRI scanner University of Cambridge A custom built PET/MRI scanner22 was used to perform the PET, its PET performance similar to an F120 micropet scanner23
Segment software Medviso, Lund University Freely available
SPM-mouse University of Cambridge Freely available

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References

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रोधगलन के माउस मॉडल में एमआरआई और पीईटी
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Cite this Article

Buonincontri, G., Methner, C.,… More

Buonincontri, G., Methner, C., Carpenter, T. A., Hawkes, R. C., Sawiak, S. J., Krieg, T. MRI and PET in Mouse Models of Myocardial Infarction. J. Vis. Exp. (82), e50806, doi:10.3791/50806 (2013).

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