Abstract
心肌梗死是导致死亡的，在西方世界的主要原因之一。小鼠心脏对人的心脏的相似性使得它用于测试新的治疗策略的理想模型。
体内磁共振成像（MRI）给出了心脏的美景无创性有明确的解剖细节，可用于精确功能评估。造影剂可提供组织活力的基本措施，但这些都是非特异性的。正电子发射断层扫描（PET）是一种补充技术，它是高度特异性的分子成像，但缺乏MRI的解剖细节。一起使用，这些技术提供了一个敏感，特异和定量工具，用于疾病和恢复心脏的治疗后的评估。
在本文中我们将介绍这些方法是如何在急性心肌梗死小鼠模型进行的。该程序描述ħERE被设计为假定的保护药物治疗的评估。我们使用磁共振成像测量收缩功能和心肌梗死面积与后期钆增强和PET与氟脱氧葡萄糖（FDG），以评估在梗死区的代谢功能。本文侧重于实用方面，如切片规划，准确的门，药物释放，图像分割，和多式联运配准。这里介绍的方法，可以实现良好的重复性和精度保持较高的吞吐量。
Materials
| Name | Company | Catalog Number | Comments |
| Gadovist | Bayer Schering Pharma | PL 00010/0535 | |
| FDG | IBA Molecular | ||
| Equipment | |||
| Bruker BioSpec 47/40 | Bruker | ||
| Bruker mouse bed | Bruker | This bed includes tubing for anesthesia delivery and scavenging. | |
| 12 cm diameter birdcage transmitter | Bruker | T5346 | |
| 2 cm diameter surface coil receiver | Bruker | T7027 | |
| Red Dot Neonatal monitoring electrodes | 3M | P/N: 2330 | |
| Monitoring equipment | SA Systems | The monitoring kit includes respiratory pillow and rectal probe for temperature measurements. | |
| Anesthesia equipment | General Anesthetic Services | ||
| Induction box | Vet Tech Solutions LTD | ||
| Cambridge split-magnet PET/MRI scanner | University of Cambridge | A custom built PET/MRI scanner22 was used to perform the PET, its PET performance similar to an F120 micropet scanner23 | |
| Segment software | Medviso, Lund University | Freely available | |
| SPM-mouse | University of Cambridge | Freely available |
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