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Biology

Tek hücreli Yara Şifa sırasında Sitoplazmik Akışı görselleştirme Published: December 19, 2013 doi: 10.3791/50848
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1,2, 1,3, 1,4, 1,5, 1,2
1Physiology Course, Marine Biological Laboratory, 2Department of Biochemistry & Biophysics, University of California San Francisco, 3Department of Physics and Astronomy, Vrije Universiteit Amsterdam, 4Max Planck Institute of Molecular Cell Biology and Genetics, 5Department of Physics, University of Illinois Urbana-Champaign

Materials

Name Company Catalog Number Comments
Stentor coeruleus live culture Carolina Biological 131598
Methyl Cellulose, 1,500 cP Sigma M0387

EQUIPMENT:
Material Name Company Catalogue Number
Soft glass stirring rod 5 in long Fisher Scientific 11-380A
Culture Dish 4.5 in 250 ml glass Carolina Biological 741004
IX81 inverted microscope Olympus
Axiovert 200M inverted microscope Zeiss
Stemi 2000 Stereo Microscope Zeiss
Evolve 512 x 512 EMCCD camera Photometrics
Plan Apo 20X objective Olympus UPLSAPO

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References

  1. Sonnemann, K. J., Bement, W. M. Wound repair: toward understanding and integration of single-cell and multicellular wound responses. Annu. Rev. Cell Dev. Biol. 27, 237-264 (2011).
  2. Idone, V., Tam, C., Andrews, N. W. Two-way traffic on the road to plasma membrane repair. Trends Cell Biol. 18 (11), 552-559 (2008).
  3. Reddy, A., Caler, E. V., Andrews, N. W. Plasma membrane repair is mediated by Ca2+-regulated exocytosis of lysosomes. Cell. 106 (2), 157-169 (2001).
  4. Foissner, I., Wastenays, G. O. The characean internodal cell as a model system for studying wound healing. J. Microsc. 247 (1), 10-12 (2012).
  5. Bement, W. M., Mandato, C. A., Kirsch, M. N. Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes. Curr. Biol. 9 (11), 579-587 (1999).
  6. Kim, H. G., Klotchkova, T. A., Kang, Y. M. Life without a cell membrane: regeneration of protoplasts from disintegrated cells of the marine green alga Bryopsis plumosa. J. Cell Sci. 114 (11), 2009-2014 (2001).
  7. Tartar, V. The Biology of Stentor. , Pergamon Press. 434 (1961).
  8. Morgan, T. H. Regeneration of proportionate structures in Stentor. Biol. Bull. 2 (6), 311-328 (1901).
  9. Heidemann, S. R., Wirtz, D. Towards a regional approach to cell mechanics. Trends Cell Biol. 14 (4), 160-166 (2004).
  10. Kasza, K. E., Rowat, A. C., Liu, J., Angelini, T. E., Brangwynne, C. P., Koenderinck, G. H., Weitz, D. A. The cell as a material. Curr. Opin. Cell Biol. 19 (1), 101-107 (2007).
  11. Mitchison, T. J., Charras, G. T., Mahadevan, L. Implications of a poroelastic cytoplasm for the dynamics of animal cell shape. Sem. Cell Dev. Biol. 19 (3), 215-223 (2008).
  12. De Terra, N. Culture of Stentor coeruleus on Colpidium campylum. J. Eukar. Microbiol. 13 (3), 491-492 (1966).
  13. Harris, E. H. The Chlamydomonas Sourcebok. 1, (2009).
  14. Vallotton, P., Ponti, A., Waterman-Storer, C. M., Salmon, E. D., Danuser, G. Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. Biophys. J. 85 (2), 1289-1306 (2003).
  15. Miyake, A., Harumoto, T., Iio, H. Defence function of pigment granules in Stentor coeruleus. Eur. J. Protistol. 37 (1), 7-88 (2001).
Tek hücreli Yara Şifa sırasında Sitoplazmik Akışı görselleştirme<em&gt; Stentor coeruleus</em
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Cite this Article

Slabodnick, M., Prevo, B., Gross,… More

Slabodnick, M., Prevo, B., Gross, P., Sheung, J., Marshall, W. Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus. J. Vis. Exp. (82), e50848, doi:10.3791/50848 (2013).

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