Abstract
タンパク質は、それらの特異的な三次元フォールドの誘発機能を発揮することによって生物で重要なタスクを実行する。ポリペプチドの天然の構造は、多くの目的を果たすが、それは現在ほとんどのタンパク質は、β-シートリッチアミロイドの代替的なアセンブリを採用することができることが認識される。不溶性のアミロイド線維は、最初は複数のヒトの病気と関連しているが、それらは、ますます様々な重要な細胞プロセスに関与する官能選手として表示されます。加えて、患者の組織において沈着したアミロイドは、そのような核酸およびグリコサミノグリカン（GAG）のような非タンパク質性成分を含む。これらの補因子は、不溶性沈殿物の異なるタイプの生成をもたらす、アミロイドの形成を容易にすることができる。タンパク質は、可溶性アミロイド前駆体の中間段階を介して誤って折り畳ま方法我々の理解を活用することにより、我々は、in vitroでアミロイド原繊維への天然タンパク質に変換する方法を考案した
Materials
| Name | Company | Catalog Number | Comments |
| 2-(N-morpholino)ethanesulfonic acid (MES) | Sigma-Aldrich | M8250 | |
| NaCl | Fisher | BP358-10 | |
| 1-ethyl-3-[3-dimethyl-aminopropyl] carbodiimide hydrochloride (EDC) | Thermo/Pierce | 22980 | |
| DNA from salmon sperm | Sigma | D1626 | |
| RNA from torula yeast | Sigma | R6625 | |
| Heparin from porcine intestinal mucosa | Sigma | H3149 | |
| Tris base | Fisher | BP152-1 | |
| Equipment: | |||
| Water bath | Fisher Science | Isotemp 210 | |
| Slide-A-Lyzer dialysis cassette | Thermo/Pierce | 66380 |
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