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Biology

천연 단백질에서 아밀로이드의 빠른 생성 Published: December 5, 2013 doi: 10.3791/50869

Materials

Name Company Catalog Number Comments
2-(N-morpholino)ethanesulfonic acid (MES) Sigma-Aldrich M8250
NaCl Fisher BP358-10
1-ethyl-3-[3-dimethyl-aminopropyl] carbodiimide hydrochloride (EDC) Thermo/Pierce 22980
DNA from salmon sperm Sigma D1626
RNA from torula yeast Sigma R6625
Heparin from porcine intestinal mucosa Sigma H3149
Tris base Fisher BP152-1
Equipment:
Water bath Fisher Science Isotemp 210
Slide-A-Lyzer dialysis cassette Thermo/Pierce 66380

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References

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  20. Vetri, V., et al. Bovine Serum Albumin protofibril-like aggregates formation: Solo but not simple mechanism. Arch. Biochem. Biophysics. 508, 13-24 (2011).
  21. Bucciantini, M., et al. Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases. Nature. 416, 507-511 (2002).
  22. McParland, V. J., Kalverda, A. P., Homans, S. W., Radford, S. E. Structural properties of an amyloid precursor of b2-microglobulin. Nat. Struct. Mol. Biol. 9, 326-331 (2002).
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천연 단백질에서 아밀로이드의 빠른 생성<em&gt; 체외</em
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Cite this Article

Dorta-Estremera, S. M., Li, J., Cao, More

Dorta-Estremera, S. M., Li, J., Cao, W. Rapid Generation of Amyloid from Native Proteins In vitro. J. Vis. Exp. (82), e50869, doi:10.3791/50869 (2013).

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