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JoVE Journal Immunology and Infection
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Immunology and Infection

Whole-celle MALDI-TOF massespektrometri er en nøyaktig og rask metode for å analysere forskjellige moduser av Macrophage Aktivisering

Published: December 26, 2013 doi: 10.3791/50926
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1, 1,2, 1, 1, 1
1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095, Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique, Hôpital de la Timone

Abstract

MALDI-TOF er en mye brukt massespektrometri teknikk i kjemi og biokjemi. Det har også blitt anvendt i medisinen for å identifisere molekyler og biomarkører. Nylig har det vært brukt i mikrobiologi for rutinen identifisering av bakterier vokst fra kliniske prøver, uten forberedelse eller fraksjoneringstrinn. Vi og andre har brukt denne hel-celle MALDI-TOF massespektrometri teknikken med hell til eukaryote celler. Aktuelle søknader spenner fra celletype identifikasjon til kvalitetskontroll vurdering av cellekultur og diagnostiske applikasjoner. Her beskriver vi bruken for å utforske de forskjellige polarisasjons-fenotypene av makrofager som respons på cytokiner eller varmedrepte bakterier. Det tillates identifisering av makrofager spesifikke fingeravtrykk som er representative for mangfoldet av proteomikk svar av makrofager. Denne søknaden illustrerer nøyaktighet og enkelhet ved fremgangsmåten. Protokollen vi beskrevet her kan være nyttig for studere immunrespons i verten patologiske tilstander eller kan bli utvidet til bredere diagnostiske anvendelser.

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Whole-celle MALDI-TOF massespektrometri er en nøyaktig og rask metode for å analysere forskjellige moduser av Macrophage Aktivisering
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Ouedraogo, R., Daumas, A., Capo, C., … More

Ouedraogo, R., Daumas, A., Capo, C., Mege, J. L., Textoris, J. Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation. J. Vis. Exp. (82), e50926, doi:10.3791/50926 (2013).

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