November 2013: This Month in JoVE

Summary

Here's a look at what's coming up in the November 2013 issue of JoVE.

Protocol

Pre-hospital Thrombolysis: A Manual from Berlin

Martin Ebinger^1,2,5, Sascha Lindenlaub^1,3,5, Alexander Kunz^1,2,5, Michal Rozanski^2,5, Carolin Waldschmidt^2,5, Joachim E. Weber^2,5, Matthias Wendt^2,5, Benjamin Winter^1,2,5, Philipp A. Kellner^4,5, Sabina Kaczmarek^4,5, Matthias Endres^1,2,5, Heinrich J. Audebert^1,2,5

¹Center for Stroke Research Berlin (CSB), Charité - Universitätsmedizin Berlin, ²Klinik und Hochschulambulanz für Neurologie, Charité - Universitätsmedizin Berlin, ³Medical School of the Universität Hamburg, Universitätsklinikum Hamburg - Eppendorf, ⁴Berliner Feuerwehr, ⁵STEMO-Consortium

Identification of suspected stroke in the dispatch center of the Berlin Fire Brigade prompts the deployment of a CT-equipped ambulance. If ischemic stroke is confirmed and contraindications are excluded pre-hospital thrombolysis is applied.

Neural Circuit Recording from an Intact Cockroach Nervous System

Josh S. Titlow¹, Zana R. Majeed^1,2, H. Bernard Hartman³, Ellen Burns¹, Robin L. Cooper¹

¹Department of Biology, University of Kentucky, ²Department of Biology, University of Salahaddin, ³Oregon Institute of Marine Biology, University of Oregon

This article describes the cockroach ventral nerve cord dissection and extracellular recordings from the cercal nerve and connectives. Evoked responses are generated by electrical stimulation of the cercal nerve or direct mechanical stimulation of the cerci.

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

Josh Titlow¹, Zana R. Majeed^1,2, John G Nicholls³, Robin L. Cooper¹

¹Department of Biology, University of Kentucky, ²Department of Biology, College of Science, University of Salahaddin, Iraq, ³Department of Neurobiology and Cognitive Neuroscience, SISSA, Italy

This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.

Simulation of the Planetary Interior Differentiation Processes in the Laboratory

Yingwei Fei

Geophysical Laboratory, Carnegie Institution of Washington

The high-pressure and high-temperature experiments described here mimic planet interior differentiation processes. The processes are visualized and better understood by high-resolution 3D imaging and quantitative chemical analysis.