Summary
Here's a look at what's coming up in the December 2013 issue of JoVE.
Protocol
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup and Operation
Alexander Gruenberger, Christopher Probst, Antonia Heyer, Wolfgang Wiechert, Julia Frunkze, Dietrich Kohlheyer
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
Surface Renewal: An Advanced Micrometeorological Method for Measuring and Processing Field-Scale Energy Flux Density Data
Andrew J. McElrone1,2, Thomas M. Shapland2, Arturo Calderon2,3, Li Fitzmaurice4,5, Kyaw Tha Paw U4, Richard L. Snyder4
1Crops Pathology and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, 2Department of Viticulture & Enology, University of California, Davis, 3Faculty of Agricultural Sciences, University of Chile, 4Atmospheric Science, University of California, Davis, 5URS Corporation Australia Pty. Ltd.
Surface renewal is a micrometeorological method that is being used increasingly to determine energy fluxes, but its technical complexity makes it inaccessible to a broad audience. We describe the steps needed to set up and calibrate a surface renewal field station, to acquire and process data, and to correctly interpret results.
Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes
Sandy Rottloff1, Axel Mithöfer2, Ute Müller3, Roland Kilper3
1Laboratoire Agronomie et Environnement, Université de Lorraine, 2Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, 3aura optik
Plants glands are specialized structures responsible for the biosynthesis and secretion of many compounds involved in interactions with the biotic environment. To enable studies on their molecular and biochemical features, a mechanical micropreparation technique was established in order to isolate single metabolically active glands, here from the carnivorous plant Nepenthes.
Dual-phase Cone-beam Computed Tomography to See, Reach, and Treat Hepatocellular Carcinoma during Drug-eluting Beads Transarterial Chemo-embolization
Vania Tacher1, MingDe Lin2, Nikhil Bhagat1, Nadine Abi Jaoudeh3, Alessandro Radaelli4, Niels Noordhoek4, Bart Carelsen4, Bradford J. Wood3, Jean-François Geschwind1
1Russell H. Morgan Department of Radiology and Radiological Science, Division of Vascular and Interventional Radiology, The Johns Hopkins Hospital, 2Clinical Informatics, Interventional, and Translational Solutions (CIITS), Philips Research North America, 3Center for Interventional Oncology, Interventional Radiology Section, National Institutes of Health, 4Interventional X-ray, Philips Healthcare
Dual-phase cone-beam computed tomography (DP-CBCT) is a useful intraprocedural imaging technique for transarterial chemo-embolization treatment with drug-eluting beads of hepatocellular carcinoma. DP-CBCT has been used to perform three major steps in oncologic interventional radiology: tumor localization (see), navigation and intraprocedural catheter guidance (reach), and intraprocedural evaluation of treatment success (treat).
High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
Laura Ekstrand, Nikolaus Karpinsky, Yajun Wang, Song Zhang
3D Machine Vision Laboratory, Department of Mechanical Engineering, Iowa State University
This video describes the fundamentals of digital fringe projection techniques, which provide dense 3D measurements of dynamically changing surfaces. It also demonstrates the design and operation of a high-speed binary defocusing system based on these techniques.
Disclosures
No conflicts of interest declared.