Materials
Name | Company | Catalog Number | Comments |
BubbleStop Syringe Heater | AutoMate Scientific | 10-4-35-G | |
CaCl2 | Sigma | C3306 | |
Collagenase, Type II, powder | Gibco | 17101-015 | |
Dispase | Sigma-Aldrich | 42613-33-2 | |
Dissection tools | Roboz | ||
DMSO | Sigma-Aldrich | D5879 | |
Fixed-stage microscope | Olympus | BX51WI | |
Fluo-4 AM dye | Invitrogen | F-14201 | |
Glucose | Sigma | G8270 | |
Insect pins | Fine Science Tools | Minutien Pins | |
iQ Live Cell Imaging Software | Andor | Andor iQ3 | |
KCl | Sigma | P3911 | |
MgCl2 | Sigma | M9272 | |
NaCl | Sigma | S9888 | |
NaH2PO4 | Sigma | S8282 | |
NaHCO3 | Sigma | S6014 | |
Neo sCMOS camera | Andor | Neo 5.5 sCMOS | |
Nicardipine | Sigma | N7510 | |
Perfusion chamber | Custom | ||
Peristaltic pump | Harvard Apparatus | Model 720 | |
Pluronic F-127 | Invitrogen | P3000MP | |
Probenecid | Molecular Probes | P36400 | |
Scopolamine | Sigma | S1013 | |
Sutter Lambda DG-4 | Sutter | DG-4 | |
Sylgard | Dow Corning | 184 | |
Temperature Controller | Warner Instruments | TC-344C |
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