Summary
Here's a look at what's coming up in the March 2014 issue of JoVE.
Protocol
Study of Phagolysosome Biogenesis in Live Macrophages
Marc Bronietzki1, Bahram Kasmapour1, Maximiliano Gabriel Gutierrez1,2
1Research Group Phagosome Biology, Helmholtz Centre for Infection Research, 2Division of Mycobacterial Research, National Institute for Medical Research
Studying Food Reward and Motivation in Humans
Hisham Ziauddeen1,2,3, Naresh Subramaniam1, Victoria C. Cambridge4, Nenad Medic1,2, Ismaa Sadaf Farooqi2, Paul C. Fletcher1,2,3
1Department of Psychiatry, University of Cambridge, 2Metabolic Research Laboratories, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, 3Cambridgeshire & Peterborough NHS Foundation Trust, University of Cambridge, 4West Anglia Comprehensive Local Research Network, Addenbrooke's Hospital
This article describes a set of methods for the measurement of food related motivation and food related goal values in humans.
Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
Mark A. Hughes1, Paul M. Brennan2, Andrew S. Bunting3, Mike J. Shipston1, Alan F. Murray3
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh
This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.
Disclosures
No conflicts of interest declared.